Application of next-generation sequencing to characterize novel mutations in clarithromycin-susceptible Helicobacter pylori strains with A2143G of 23S rRNA gene

Table 4 The special variations were identified in CLA-resistant strain with A2143G

Gene	Position of mutation^a	Ref	Mutation	Amino acid	Annotation	Mean depth
HP0254	265	G	A	G89S	Outer membrane protein HopG	465.5
rpsA	482	G	A	G161D	30S ribosomal protein S1	543.5
mtrC	542	C	T	T181I	Membrane fusion protein	525.5
HP0498	744	T	G	F248L	Sodium⁻ and chloride⁻ dependent transporter	597.5
proWX	1650	A	C	R550S	Osmoprotectant ABC transporter permease	642
HP0853	943	C	A	R315S	ABC transporter ATP-binding protein	441.5
hypD	474	G	A (stop)	W158X	Hydrogenase maturation factor	512
hefD	13	G	T	G5C	Outer membrane protein	583.5
hefD	82	A	G	M28V	Outer membrane protein	346
tolB	210	T	G	D70E	Translocation protein	705.5
rpsP	226	G	A	A76T	30S ribosomal protein S16	650
HP1181	826	A	G	I276V	Multidrug transporter	506
rplA	464	G	A	S155N	50S ribosomal protein L16	561.5
HP1220	661	G	A	A221T	ABC transporter ATP-binding protein	628
HP1220	662	C	T	A221V	ABC transporter ATP-binding protein	624.5
moaA	76	C	T (stop)	Q26X	Molybdenum cofactor biosynthesis protein A	528.5

^aNucleotide position was determined by the respective reference sequence from NC_000915

ISSN: 1476-0711